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Antimicrobial activities of halicin (MIC, μg/mL).
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Antimicrobial activities of halicin (MIC, μg/mL).
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Microglial DICER protein is specifically downregulated in the MPTP model. a , b Upper—representative immunoblots of total lysates from the primary cultured microglia incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER ( a <t>),</t> <t>Ago2,</t> and <t>Drosha</t> ( b ). Lower—statistics ( n = 4 for DICER, n = 5 for Ago2, and n = 4 for Drosha). c Upper—representative immunoblots of DICER from mouse neurons or astrocytes treated with the indicated concentrations of MPP + for 12 h. Lower—statistics ( n = 4 for neurons, n = 6 for astrocytes). d Upper—representative immunoblots of total lysates from the BV2 cells incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER, Ago2, and Drosha. Lower—statistics ( n = 5 for DICER and n = 4 for Ago2 and Drosha). e Left—representative immunoblots of total lysates from the CD11b+/CD45low-labeled microglia sorted from mouse ventral mesencephalon (VM) by flow cytometric 3 days after the administration of saline or MPTP and probed with the antibodies for DICER, Ago2, and Drosha. Right—statistics ( n = 4 for DICER, Ago2, and Drosha). f Upper—representative immunoblots of total lysates from the GLAST+ labeled astrocytes sorted from VM by flow cytometric 3 days after administration of saline or MPTP and probed with the DICER antibody. Lower—statistics ( n = 4 for DICER). Data are mean + SEM. * p < 0.05, ** p < 0.01. Unless stated, α-tubulin (α-Tub) or Actin as loading controls. kDa, kilodalton
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Microglial DICER protein is specifically downregulated in the MPTP model. a , b Upper—representative immunoblots of total lysates from the primary cultured microglia incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER ( a <t>),</t> <t>Ago2,</t> and <t>Drosha</t> ( b ). Lower—statistics ( n = 4 for DICER, n = 5 for Ago2, and n = 4 for Drosha). c Upper—representative immunoblots of DICER from mouse neurons or astrocytes treated with the indicated concentrations of MPP + for 12 h. Lower—statistics ( n = 4 for neurons, n = 6 for astrocytes). d Upper—representative immunoblots of total lysates from the BV2 cells incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER, Ago2, and Drosha. Lower—statistics ( n = 5 for DICER and n = 4 for Ago2 and Drosha). e Left—representative immunoblots of total lysates from the CD11b+/CD45low-labeled microglia sorted from mouse ventral mesencephalon (VM) by flow cytometric 3 days after the administration of saline or MPTP and probed with the antibodies for DICER, Ago2, and Drosha. Right—statistics ( n = 4 for DICER, Ago2, and Drosha). f Upper—representative immunoblots of total lysates from the GLAST+ labeled astrocytes sorted from VM by flow cytometric 3 days after administration of saline or MPTP and probed with the DICER antibody. Lower—statistics ( n = 4 for DICER). Data are mean + SEM. * p < 0.05, ** p < 0.01. Unless stated, α-tubulin (α-Tub) or Actin as loading controls. kDa, kilodalton
B Gallinarum Atcc 33778 Nt B Indicum Dsm, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Antimicrobial activities of halicin (MIC, μg/mL).

Journal: Frontiers in Pharmacology

Article Title: Safety and efficacy evaluation of halicin as an effective drug for inhibiting intestinal infections

doi: 10.3389/fphar.2024.1389293

Figure Lengend Snippet: Antimicrobial activities of halicin (MIC, μg/mL).

Article Snippet: Salmonella (CVCC 3377) , 8 , E. faecalis (ATCC 29212) , 4.

Techniques:

Growth kinetics curve assays of resistant Salmonella (A) , Proteus (B) , Klebsiella pneumoniae (C) , S. aureus (D) , E. coli (E) , SAR ( S. aureus ATCC 29213 resistant strains) (F) and ECR ( E. coli ATCC 25922 resistant strains) (G) .

Journal: Frontiers in Pharmacology

Article Title: Safety and efficacy evaluation of halicin as an effective drug for inhibiting intestinal infections

doi: 10.3389/fphar.2024.1389293

Figure Lengend Snippet: Growth kinetics curve assays of resistant Salmonella (A) , Proteus (B) , Klebsiella pneumoniae (C) , S. aureus (D) , E. coli (E) , SAR ( S. aureus ATCC 29213 resistant strains) (F) and ECR ( E. coli ATCC 25922 resistant strains) (G) .

Article Snippet: Salmonella (CVCC 3377) , 8 , E. faecalis (ATCC 29212) , 4.

Techniques:

Ames test results of halicin using  Salmonella  typhimurium strains TA97, TA98, TA100, and WP2uvrApKM101.

Journal: Frontiers in Pharmacology

Article Title: Safety and efficacy evaluation of halicin as an effective drug for inhibiting intestinal infections

doi: 10.3389/fphar.2024.1389293

Figure Lengend Snippet: Ames test results of halicin using Salmonella typhimurium strains TA97, TA98, TA100, and WP2uvrApKM101.

Article Snippet: Salmonella (CVCC 3377) , 8 , E. faecalis (ATCC 29212) , 4.

Techniques: Ames Test, Control

Microglial DICER protein is specifically downregulated in the MPTP model. a , b Upper—representative immunoblots of total lysates from the primary cultured microglia incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER ( a ), Ago2, and Drosha ( b ). Lower—statistics ( n = 4 for DICER, n = 5 for Ago2, and n = 4 for Drosha). c Upper—representative immunoblots of DICER from mouse neurons or astrocytes treated with the indicated concentrations of MPP + for 12 h. Lower—statistics ( n = 4 for neurons, n = 6 for astrocytes). d Upper—representative immunoblots of total lysates from the BV2 cells incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER, Ago2, and Drosha. Lower—statistics ( n = 5 for DICER and n = 4 for Ago2 and Drosha). e Left—representative immunoblots of total lysates from the CD11b+/CD45low-labeled microglia sorted from mouse ventral mesencephalon (VM) by flow cytometric 3 days after the administration of saline or MPTP and probed with the antibodies for DICER, Ago2, and Drosha. Right—statistics ( n = 4 for DICER, Ago2, and Drosha). f Upper—representative immunoblots of total lysates from the GLAST+ labeled astrocytes sorted from VM by flow cytometric 3 days after administration of saline or MPTP and probed with the DICER antibody. Lower—statistics ( n = 4 for DICER). Data are mean + SEM. * p < 0.05, ** p < 0.01. Unless stated, α-tubulin (α-Tub) or Actin as loading controls. kDa, kilodalton

Journal: Journal of Neuroinflammation

Article Title: JNK-mediated microglial DICER degradation potentiates inflammatory responses to induce dopaminergic neuron loss

doi: 10.1186/s12974-018-1218-1

Figure Lengend Snippet: Microglial DICER protein is specifically downregulated in the MPTP model. a , b Upper—representative immunoblots of total lysates from the primary cultured microglia incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER ( a ), Ago2, and Drosha ( b ). Lower—statistics ( n = 4 for DICER, n = 5 for Ago2, and n = 4 for Drosha). c Upper—representative immunoblots of DICER from mouse neurons or astrocytes treated with the indicated concentrations of MPP + for 12 h. Lower—statistics ( n = 4 for neurons, n = 6 for astrocytes). d Upper—representative immunoblots of total lysates from the BV2 cells incubated with the indicated concentrations of MPP + for 12 h and probed with the antibodies for DICER, Ago2, and Drosha. Lower—statistics ( n = 5 for DICER and n = 4 for Ago2 and Drosha). e Left—representative immunoblots of total lysates from the CD11b+/CD45low-labeled microglia sorted from mouse ventral mesencephalon (VM) by flow cytometric 3 days after the administration of saline or MPTP and probed with the antibodies for DICER, Ago2, and Drosha. Right—statistics ( n = 4 for DICER, Ago2, and Drosha). f Upper—representative immunoblots of total lysates from the GLAST+ labeled astrocytes sorted from VM by flow cytometric 3 days after administration of saline or MPTP and probed with the DICER antibody. Lower—statistics ( n = 4 for DICER). Data are mean + SEM. * p < 0.05, ** p < 0.01. Unless stated, α-tubulin (α-Tub) or Actin as loading controls. kDa, kilodalton

Article Snippet: The following antibodies were used: TH (AB152) and spectrin (#Mab 1622) from Millipore; Iba1 (#01919741) from Wako; HA (H6908) and GFAP (G3893) from Sigma; Ago2 (sc-32877), Drosha (sc-33778), DICER (sc-30226), ubquitin (sc-8017), phosphorylated JNK (sc-6254), and JNK (sc-7345) from Santa Cruz Biotechnology; Caspase-3 (9662), cleaved Caspase-3 (#9664 L), ERK1/2 (9102), phosphorylated ERK (9101S), phosphorylated p38 (9216S), and p38 (8690S) from Cell Signaling Technology; phospho Serine (37430) from Qiagen; CD11b-Alexa Fluor 488 (557672) and CD45-APC (559864) from BD Biosciences; and GLAST-APC (130-098-803) from MiltenyiBiotec.

Techniques: Western Blot, Cell Culture, Incubation, Labeling, Saline